baculoviral IAP repeat containing 5(BIRC5) Homo sapiens. This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011],
Catalog No
N1515
Reactivity
Human,Rat
Applications
WB,IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:1000-2000 IHC: 1:200-500
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1. Using Survivin Mouse mAb (N1515) diluted at 1:200 (4° overnight). 2. High-pressure and temperature Citric acid. pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
Western blot detection of Survivin in human breast cancer cell line MCF-7(A) and Cal51 (B) using Survivin mouse mAb (N1515. 1:1000 diluted).Predicted band size: 16kDa.Observed band size:16kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1.Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1.Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
