signal transducer and activator of transcription 3(STAT3)Homo sapiens. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper
Catalog No
N1544
Reactivity
Human,Rat,Mouse
Applications
WB,IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB 1:1000-2000, IHC 1:100-200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Molecular Weight (Da)
Gene Name
STAT3
Protein Name
Signal transducer and activator of transcription 3 (Acute-phase response factor)
Human Gene ID
6774
Human Swiss Prot No.
P40763
Immunogen
Recombinant Protein of STAT3
Specificity
STAT3 protein detects endogenous levels of STAT3
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot detection of STAT3 in human breast cancer cell line MCF-7(A). T47D(B). MDA-MB-231(C) and Cal51 (D) using STAT3 mouse mAb (N1544. 1:2000 diluted).Predicted band size: 86kDa.Observed band size:86kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
