platelet derived growth factor receptor alpha(PDGFRA) Homo sapiens. This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012],
Catalog No
N1662
Reactivity
Human,Rat,Mouse
Applications
IF/ICC,IHC-p
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
IF: 1:50-200 IHC 1:100-200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PDGFRA
Molecular Weight (Da)
Gene Name
PDGFRA
Protein Name
PDGFRA
Human Gene ID
5156
Human Swiss Prot No.
P16234
Immunogen
Synthetic Peptide of PDGFRα at AA range of 1010-1090
Specificity
PDGFRα protein detects endogenous levels of PDGFRA
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1.PDGFRα Mouse Monoclonal Antibody(7A3) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
