Galectin-3 is a member of the β-galactose binding lectin family with a molecular weight of 30 kDa which is a chimeric structure consisting of a β-galactoside binding protein and a laminin. The main role of Galectin-3 on the cell surface is related to the adhesion between similar cells. It is expressed in a variety of organs and tissues, including neutrophils and vascular endothelial cells, but it is obviously absent in normal hepatocytes. Galectin-3 can downregulate the expression of some cancers and breast cancer associated proteins, and promote tumor metastasis. It can be used as a reference index for the degree of tumor deterioration.
The antibody can specifically recognize human Galectin 3 protein. In western blotting of Hela, A431 and MCF7 cell lysates, the antibody can label a 30 kDa band corresponding to Galectin 3.
Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Galectin-3 antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.
Predicted band size: 26(30) kDa
Observed band size: 33 kDa
