α- Catenin、 β- Catenin、 γ- catenin and p120 catenin are members of the connexin family and mediate intercellular adhesion together with E-cadherin. P120 catenin binds to the near membrane region of E-cadherin to form a complex, which is stable and closely linked. In lobular breast cancer, E-cadherin deficiency resulted in the accumulation of p120 catenin in the cytoplasm, while in ductal breast cancer, p120 catenin in the membrane decreased and there was no accumulation of p120 catenin in the cytoplasm. Therefore, it can be used to distinguish lobular carcinoma from ductal carcinoma. Studies have shown that p120 catenin in the cytoplasm of gastric and colon cancer is related to poor adhesion of cancer cells.
The antibody can specifically recognize human p120 catenin protein. In western blotting of Hela, A431 and HEK293 cell lysates, the antibody can label a band with molecular weight around 100~110 kDa.
Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-p120 catenin antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.
Predicted band size: 108 kDa
