Cadherin is a kind of adhesion protein which belongs to a transmembrane glycoprotein superfamily. It plays an important role in structural integrity of epithelia. This antibody can detect many isotypes of cadherin.
The antibody can recognize many isotypes of cadherin including E-cadherin, N-cadherin, P-cadherin and R-cadherin. In western blotting of A431 and MCF7 cell lysates, the antibody can label a 130 kDa band.
A431 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-cadherin(ABT284)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.
Lane 1: A431
Predicted band size: 125-140kDa
Observed band size: 120,150kDa