The p73 (Acetyl-Lys327) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect p73 (Acetyl-Lys327) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of p73 (Acetyl-Lys327) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on p73 (Acetyl-Lys327) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1085
Format
Two 96-Well Plates
Detection Target
p73 (Acetyl-Lys327) Antibody
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
TP73
NCBI Gene ID
7161
Target Synonyms
TP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein
Western Blot analysis of A549 cells using p73 Polyclonal Antibody. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).