The Rb (Acetyl-K873/K874) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Rb (Acetyl-K873/K874) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of Rb (Acetyl-K873/K874) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Rb (Acetyl-K873/K874) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1078
Format
Two 96-Well Plates
Detection Target
Rb (Acetyl-K873/K874) Antibody
Reactivity
Human:K873+K874, Mouse:K866+K867, Rat:K865+K866
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
RB1
NCBI Gene ID
5925
Target Synonyms
Retinoblastoma-associated protein (p105-Rb) (pRb) (Rb) (pp110)
Western blot analysis of 293T using RB1 antibody. Antibody was diluted at 1:500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)