The HMG-1 (Acetyl-Lys12) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect HMG-1 (Acetyl-Lys12) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of HMG-1 (Acetyl-Lys12) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on HMG-1 (Acetyl-Lys12) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1076
Format
Two 96-Well Plates
Detection Target
HMG-1 (Acetyl-Lys12) Antibody
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
HMGB1
NCBI Gene ID
3146
Target Synonyms
HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1
Western Blot analysis of HeLa cells using HMG-1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western Blot analysis of HepG2 cells treated with UV using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000