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PARP-1 (Acetyl-K521) Colorimetric Cell-Based ELISA Kit
PARP-1 (Acetyl-K521) Colorimetric Cell-Based ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: CBA1075
Reactivity:Human:K521,Mouse:K520,Rat:K521
Assay Type:Cell-Based ELISA
Format:Two 96-Well Plates
Range:At least 5000 cells/well
Pairing Option:Non-Acetyl Antibody

$500
# Datasheet #
# SDS #
Crystal Violet Normalization
Customizable Format
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PARP-1 (Acetyl-K521) Colorimetric Cell-Based ELISA Kit

An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.

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Product Details
Product Name
PARP-1 (Acetyl-K521) Colorimetric Cell-Based ELISA Kit
Description
The PARP-1 (Acetyl-K521) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect PARP-1 (Acetyl-K521) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of PARP-1 (Acetyl-K521) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on PARP-1 (Acetyl-K521) Antibody. This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1075
Format
Two 96-Well Plates
Detection Target
PARP-1 (Acetyl-K521) Antibody
Reactivity
Human:K521, Mouse:K520, Rat:K521
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
PARP1 ADPRT PPOL
NCBI Gene ID
142
Target Synonyms
Poly [ADP-ribose] polymerase 1 (PARP-1) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 1) (ARTD1) (NAD(+) ADP-ribosyltransferase 1) (ADPRT 1) (Poly[ADP-ribose] synthase 1)
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Time
4.5 hours
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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