The AR(Acetyl-K631) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect AR(Acetyl-K631) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of AR(Acetyl-K631) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on AR(Acetyl-K631) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1074
Format
Two 96-Well Plates
Detection Target
AR(Acetyl-K631) Antibody
Reactivity
Human:K631, Mouse:K610, Rat:K613
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
AR DHTR NR3C4
NCBI Gene ID
367
Target Synonyms
Androgen receptor (Dihydrotestosterone receptor) (Nuclear receptor subfamily 3 group C member 4)
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
Western Blot analysis of mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)