The Ku-70 (Acetyl-Lys317) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Ku-70 (Acetyl-Lys317) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of Ku-70 (Acetyl-Lys317) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Ku-70 (Acetyl-Lys317) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1070
Format
Two 96-Well Plates
Detection Target
Ku-70 (Acetyl-Lys317) Antibody
Reactivity
Human, Rat, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
XRCC6 G22P1
NCBI Gene ID
2547
Target Synonyms
X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP lyase Ku70) (70 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Lupus Ku autoantigen protein p70) (Ku70) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 6)
