The Stat3 (Acetyl-Lys87) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Stat3 (Acetyl-Lys87) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of Stat3 (Acetyl-Lys87) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Stat3 (Acetyl-Lys87) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1050
Format
Two 96-Well Plates
Detection Target
Stat3 (Acetyl-Lys87) Antibody
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
STAT3
NCBI Gene ID
6774
Target Synonyms
STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Western blot analysis of MOUSE-SPLEEN using Acetyl-Stat3 (K87) antibody. Antibody was diluted at 1:500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000