The HNF-4α (Acetyl-Lys106) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect HNF-4α (Acetyl-Lys106) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of HNF-4α (Acetyl-Lys106) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on HNF-4α (Acetyl-Lys106) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1049
Format
Two 96-Well Plates
Detection Target
HNF-4α (Acetyl-Lys106) Antibody
Reactivity
Human, Rat, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
HNF4A HNF4 NR2A1 TCF14
NCBI Gene ID
3172
Target Synonyms
Hepatocyte nuclear factor 4-alpha (HNF-4-alpha) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) (TCF-14) (Transcription factor HNF-4)
Western blot analysis of mouse-lung mouse-kidney mouse-liver lysate, antibody was diluted at 500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
