The Histone H2B (Acetyl-Lys15) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Histone H2B (Acetyl-Lys15) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of Histone H2B (Acetyl-Lys15) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Histone H2B (Acetyl-Lys15) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1045
Format
Two 96-Well Plates
Detection Target
Histone H2B (Acetyl-Lys15) Antibody
Reactivity
Human, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
H2BFS
NCBI Gene ID
54145
Target Synonyms
H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s;H2BK15AC
Western Blot analysis of 293T cells using Histone H2B Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western Blot analysis of 3T3 cells using Acetyl-Histone H2B (K15) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000