The Ub (Acetyl-Lys29) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Ub (Acetyl-Lys29) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of Ub (Acetyl-Lys29) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Ub (Acetyl-Lys29) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1035
Format
Two 96-Well Plates
Detection Target
Ub (Acetyl-Lys29) Antibody
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
UBA52/RPS27A/UBB/UBC
NCBI Gene ID
0
Target Synonyms
UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1