The ACSS1 (Acetyl-K642) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect ACSS1 (Acetyl-K642) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of ACSS1 (Acetyl-K642) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on ACSS1 (Acetyl-K642) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBA1013
Format
Two 96-Well Plates
Detection Target
ACSS1 (Acetyl-K642) Antibody
Reactivity
Human:K642, Mouse:K635, Rat:K635
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
ACSS1 ACAS2L KIAA1846
NCBI Gene ID
84532
Target Synonyms
Acetyl-coenzyme A synthetase 2-like, mitochondrial (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) (AceCS2) (Acyl-CoA synthetase short-chain family member 1)
Western Blot analysis of 1,293T 2,hela cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
