Cleaved-Integrin α5 HC (F42) Colorimetric Cell-Based ELISA Kit
Description
The Cleaved-Integrin α5 HC (F42) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Cleaved-Integrin α5 HC (F42) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of Cleaved-Integrin α5 HC (F42) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Cleaved-Integrin α5 HC (F42) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBC1032
Format
Two 96-Well Plates
Detection Target
Cleaved-Integrin α5 HC (F42) Antibody
Reactivity
Human, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
ITGA5
NCBI Gene ID
3678
Target Synonyms
ITGA5; FNRA; Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD antigen CD49e
Western blot analysis of lysates from NIH/3T3 cells, treated with etoposide 25uM 24h, using ITGA5 (heavy chain,Cleaved-Phe42) Antibody. The lane on the right is blocked with the synthesized peptide.