The Caspase 10 (p23/17, Cleaved-Val220) Antibody Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Caspase 10 (p23/17, Cleaved-Val220) Antibody protein expression profile in cells. The kit can be used for measuring the relative amounts of Caspase 10 (p23/17, Cleaved-Val220) Antibody in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Caspase 10 (p23/17, Cleaved-Val220) Antibody.
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Western Blot analysis of extracts from Jurkat, K562 cells, using Caspase-10 Polyclonal Antibody. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Western blot analysis of Hela cell treated or untreated with 2 mM/ml camptothecin, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.