The YB-1 (Phospho-Ser102) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect YB-1 (Phospho-Ser102) protein expression profile in cells. The kit can be used for measuring the relative amounts of YB-1 (Phospho-Ser102) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on YB-1 (Phospho-Ser102).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2124
Format
Two 96-Well Plates
Detection Target
YB-1 (Phospho-Ser102)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P67809
NCBI Gene ID
4904
Target Synonyms
YBX1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using YB1 (Phospho-Ser102) Antibody. The lane on the right is blocked with the phospho peptide.