The Rad51 (Phospho-Thr309) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Rad51 (Phospho-Thr309) protein expression profile in cells. The kit can be used for measuring the relative amounts of Rad51 (Phospho-Thr309) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Rad51 (Phospho-Thr309).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2115
Format
Two 96-Well Plates
Detection Target
Rad51 (Phospho-Thr309)
Reactivity
Human, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
Q06609
NCBI Gene ID
5888
Target Synonyms
RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
Western blot analysis of lysates from NIH/3T3 cells, treated with starved 24h, using RAD51 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of HELA-UV 293T-UV using p-Rad51 (T309) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).