The Mucin 1 (Phospho-Tyr1229) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Mucin 1 (Phospho-Tyr1229) protein expression profile in cells. The kit can be used for measuring the relative amounts of Mucin 1 (Phospho-Tyr1229) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Mucin 1 (Phospho-Tyr1229).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2098
Format
Two 96-Well Plates
Detection Target
Mucin 1 (Phospho-Tyr1229)
Reactivity
Human, Rat, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P15941
NCBI Gene ID
4582
Target Synonyms
MUC1; PUM; Mucin-1; MUC-1; Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated ep
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is blocked with the phospho peptide.
