The MSK2 (Phospho-Thr568) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect MSK2 (Phospho-Thr568) protein expression profile in cells. The kit can be used for measuring the relative amounts of MSK2 (Phospho-Thr568) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on MSK2 (Phospho-Thr568).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2097
Format
Two 96-Well Plates
Detection Target
MSK2 (Phospho-Thr568)
Reactivity
Human, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
O75676
NCBI Gene ID
8986
Target Synonyms
RPS6KA4; MSK2; Ribosomal protein S6 kinase alpha-4; S6K-alpha-4; 90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; RSKB
Western blot analysis of lysates from Raw264.7 cells, treated with UV 5', using MSK2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of 293 cells using Phospho-MSK2 (T568) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).