The MDMX (Phospho-Ser367) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect MDMX (Phospho-Ser367) protein expression profile in cells. The kit can be used for measuring the relative amounts of MDMX (Phospho-Ser367) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on MDMX (Phospho-Ser367).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2092
Format
Two 96-Well Plates
Detection Target
MDMX (Phospho-Ser367)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
O15151
NCBI Gene ID
4194
Target Synonyms
MDM4; MDMX; Protein Mdm4; Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4
Western Blot analysis of 293T cells using MDMX Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MDM4 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.