The CD45 (Phospho-Ser1007) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect CD45 (Phospho-Ser1007) protein expression profile in cells. The kit can be used for measuring the relative amounts of CD45 (Phospho-Ser1007) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on CD45 (Phospho-Ser1007).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2053
Format
Two 96-Well Plates
Detection Target
CD45 (Phospho-Ser1007)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P08575
NCBI Gene ID
5788
Target Synonyms
PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45
Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 15', using CD45 (Phospho-Ser1007) Antibody. The lane on the right is blocked with the phospho peptide.
