The Parkin (Phospho-Ser131) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Parkin (Phospho-Ser131) protein expression profile in cells. The kit can be used for measuring the relative amounts of Parkin (Phospho-Ser131) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Parkin (Phospho-Ser131).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2026
Format
Two 96-Well Plates
Detection Target
Parkin (Phospho-Ser131)
Reactivity
Human, Rat, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
O60260
NCBI Gene ID
5071
Target Synonyms
PARK2; PRKN; E3 ubiquitin-protein ligase parkin; Parkinson juvenile disease protein 2; Parkinson disease protein 2