The Cdk2/Cdc2 (Phospho-Thr160) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Cdk2/Cdc2 (Phospho-Thr160) protein expression profile in cells. The kit can be used for measuring the relative amounts of Cdk2/Cdc2 (Phospho-Thr160) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Cdk2/Cdc2 (Phospho-Thr160).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2001
Format
Two 96-Well Plates
Detection Target
Cdk2/Cdc2 (Phospho-Thr160)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P24941
NCBI Gene ID
1017
Target Synonyms
CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase
