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AMPKβ1 (Phospho-Ser182) Colorimetric Cell-Based ELISA Kit
AMPKβ1 (Phospho-Ser182) Colorimetric Cell-Based ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: CBP1997
Reactivity:Human,Mouse,Rat,Monkey
Assay Type:Cell-Based ELISA
Format:Two 96-Well Plates
Range:At least 5000 cells/well
Pairing Option:Non-Phospho or Total Antibody

$500
# Datasheet #
# SDS #
Crystal Violet Normalization
Customizable Format
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AMPKβ1 (Phospho-Ser182) Colorimetric Cell-Based ELISA Kit

An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.

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Product Details
Product Name
AMPKβ1 (Phospho-Ser182) Colorimetric Cell-Based ELISA Kit
Description
The AMPKβ1 (Phospho-Ser182) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect AMPKβ1 (Phospho-Ser182) protein expression profile in cells. The kit can be used for measuring the relative amounts of AMPKβ1 (Phospho-Ser182) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on AMPKβ1 (Phospho-Ser182). This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP1997
Format
Two 96-Well Plates
Detection Target
AMPKβ1 (Phospho-Ser182)
Reactivity
Human, Mouse, Rat, Monkey
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
Q9Y478
NCBI Gene ID
5564
Target Synonyms
PRKAB1; AMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Time
4.5 hours
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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