The TRβ1 (Phospho-Ser142) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect TRβ1 (Phospho-Ser142) protein expression profile in cells. The kit can be used for measuring the relative amounts of TRβ1 (Phospho-Ser142) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on TRβ1 (Phospho-Ser142).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP1966
Format
Two 96-Well Plates
Detection Target
TRβ1 (Phospho-Ser142)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P10828
NCBI Gene ID
7068
Target Synonyms
THRB; ERBA2; NR1A2; THR1; Thyroid hormone receptor beta; Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta
Western blot analysis of lysates from LOVO cells, using Thyroid Hormone Receptor beta Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using TR-beta1 (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.