The Tfact JunB (Phospho-Ser259) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of JunB (Phospho-Ser259) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ JunB (Phospho-Ser259) DNA-Binding ELISA detects active JunB (Phospho-Ser259) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by PMA (200nM) and UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with the stabilization buffer in the Jurkat nuclear extract with PMA stimulation.