The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013]
Catalog No
A7137
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), IP, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
JUND; Transcription factor jun-D
Molecular Weight (Da)
35224
Gene Name
JUND
Protein Name
Transcription factor jun-D
Human Gene ID
3727
Human Swiss Prot No.
P17535
Immunogen
The antiserum was produced against synthesized peptide derived from human JunD around the phosphorylation site of Ser255. AA range:222-271
Specificity
Phospho-Jun D (S255) Polyclonal Antibody detects endogenous levels of Jun D protein only when phosphorylated at S255.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with Forskolin, using JunD (Phospho-Ser255) Antibody. The lane on the left is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunD (Phospho-Ser255) Antibody. The picture on the right is blocked with the phospho peptide.