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JunB (Phospho-Ser259) TFact™ DNA-Binding ELISA
JunB (Phospho-Ser259) TFact™ DNA-Binding ELISA

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: TFE-7015
Reactivity:Human,Mouse
Assay Type:DNA-Binding ELISA
Format:1 plate: 12 x 8-Well Microstrips
Dynamic Range:> 7.5 μg/mL Total Protein

$550
# Datasheet #
# SDS #
Transcription Factor Analysis
Nuclear/Cytoplasmic Lysate Compatible
Cell Lysis Components Included
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JunB (Phospho-Ser259) TFact™ DNA-Binding ELISA

The TFact™ JunB (Phospho-Ser259) DNA-Binding ELISA detects active JunB (Phospho-Ser259) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by PMA (200nM) and UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with the stabilization buffer in the Jurkat nuclear extract with PMA stimulation.

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Product Details
Product Name
JunB (Phospho-Ser259) TFact™ DNA-Binding ELISA
Description
The Tfact JunB (Phospho-Ser259) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of JunB (Phospho-Ser259) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
Catalog No
TFE-7015
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
JunB (Phospho-Ser259)
Reactivity
Human, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
DNA-Binding ELISA
SwissProt ID
P17275
NCBI Gene ID
JUNB
Target Synonyms
Transcription factor jun-B
Storage and Stability Guarantee
4°C/3 Months
Sample Type
Nuclear Lysates
Range
> 7.5 μg/mL Total Protein
Assay Time
5.5 hrs
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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