The Tfact JunD (Phospho-Ser255) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of JunD (Phospho-Ser255) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ JunD (Phospho-Ser255) DNA-Binding ELISA detects active JunD (Phospho-Ser255) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated by PMA (200nM) before harvest. The DNA-transcription factor complex is treated with the stablization buffer.