The Tfact MEF2D (Phospho-Ser444) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of MEF2D (Phospho-Ser444) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ MEF2D (Phospho-Ser444) DNA-Binding ELISA detects active MEF2D (Phospho-Ser444) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by serum (20% FBS) before harvest.