The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Catalog No
A7222
Reactivity
Human, Mouse, Rat, Monkey
Applications
IF/ICC, WB, IHC-p, IP, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84
Molecular Weight (Da)
87335
Gene Name
STAT1
Protein Name
Signal transducer and activator of transcription 1-alpha/beta
Human Gene ID
6772
Human Swiss Prot No.
P42224
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701. AA range:668-717
Specificity
Phospho-Stat1 (Y701) Polyclonal Antibody detects endogenous levels of Stat1 protein only when phosphorylated at Y701.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Feng, Qin. "Quantitative proteomic analysis reveals that Arctigenin alleviates concanavalin A-induced hepatitis through suppressing immune system and regulating autophagy." Frontiers in immunology 9 (2018): 1881.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT1 (Phospho-Tyr701) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from 1) MOUSE-BRAIN, 2) SY-SY5X,3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (cat:N1292) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.
Immunofluorescence analysis of Hela cell. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).
Author:
Dan He, Wan Dan, Qing Du, Bing-Bing Shen, Lin Chen, Liang-zi Fang, Jian-Jun Kuang, Chun-yu Tang, Ping Cai, Rong Yu, Shui-han Zhang, Jian-hua Huang