The Tfact B-Myb (Phospho-Ser577/581) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of B-Myb (Phospho-Ser577/581) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ B-Myb (Phospho-Ser577/581) DNA-Binding ELISA detects active B-Myb (Phospho-Ser577/581) in HepG2 and MCF7 Nuclear Extract. The HepG2 and MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.