The Tfact JunB TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of JunB active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ JunB DNA-Binding ELISA detects active JunB in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by UV (100J/M2).