The Tfact Estrogen Receptor-beta TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of Estrogen Receptor-beta active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ Estrogen Receptor-β DNA-Binding ELISA detects active Estrogen Receptor-β in Hela, Jurkat and MCF Nuclear Extract. The Hela, MCF and Jurkat cells were grown 3 days respectively in DMEM and RPMI 1640 with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) or directly harvested without UV stimulation.