This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, Cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
Catalog No
A0922
Reactivity
Human, Mouse, Rat
Applications
WB, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
ESR2; ESTRB; NR3A2; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2
Molecular Weight (Da)
59216
Gene Name
ESR2
Protein Name
Estrogen receptor beta
Human Gene ID
2100
Human Swiss Prot No.
Q92731
Immunogen
The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-beta around the phosphorylation site of Ser105. AA range:71-120
Specificity
Phospho-ERβ (S105) Polyclonal Antibody detects endogenous levels of ERβ protein only when phosphorylated at S105.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The picture on the right is blocked with the phospho peptide.
