The Tfact HIF-1alpha TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of HIF-1alpha active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
Catalog No
TFE-7084
Format
1 Plate: 12 x 8-Well Microstrips
Detection Target
HIF-1alpha
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
DNA-Binding ELISA
SwissProt ID
Q16665
NCBI Gene ID
HIF1A
Target Synonyms
Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A; BHLHE78; MOP1; PASD8
The TFact™ HIF-1α DNA-Binding ELISA detects active HIF-1α in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.