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Primary Antibodies
HIF-1α Antibody
HIF-1α Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: R12-2180
Reactivity:Human,Mouse,Rat
Applications:IF,WB,IHC,IP,ELISA
Modification:Unmodified/Total
Source:Polyclonal,Rabbit,IgG
Storage and Stability:Store at -20°C/1 year

$205
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HIF-1α Antibody

Immunofluorescence analysis of mouse-lung tissue. 1,HIF-1α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Product Details
Product Name
HIF-1α Antibody
Description
hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011],
Catalog No
R12-2180
Reactivity
Human, Mouse, Rat
Source
Polyclonal, Rabbit,IgG
Modification
Unmodified/Total
Applications
IF, WB, IHC, IP, ELISA
Dilution
IF 1:50-200 WB 1:500 - 1:2000.IP 1:200 IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma
Molecular Weight (Da or kDa)
Gene Name
HIF1A
Protein Name
Hypoxia-inducible factor 1-alpha
Human Gene ID
3091
Human Swiss Prot No.
Q16665
Immunogen
The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
Specificity
HIF-1α Polyclonal Antibody detects endogenous levels of HIF-1α protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (37)
Quxie Capsule Alleviates Colitis-associated Colorectal Cancer Through Modulating the Gut Microbiota and Suppressing A. fumigatus-induced Aerobic Glycolysis

Author: Available in link provided

Date of Publication : Available in link provided

Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology

Author: Available in link provided

Date of Publication : Available in link provided

Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro MICROBIAL PATHOGENESIS Fengyi Zhou, Xin Li, Xiaochi Chang, Zhihao Geng, Wenjing Hao, Jing Deng, Hai Ming Wong, Shuai Wang WB Mouse BMMs

Author: Available in link provided

Date of Publication : Available in link provided

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop WB Human 1:1000 /SW620, HCT116 Cells

Author: Available in link provided

Date of Publication : Available in link provided

Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis WB Human 1 : 1000 /SW1353 cells

Author: Available in link provided

Date of Publication : Available in link provided

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:200 retinal tissue

Author: Available in link provided

Date of Publication : Available in link provided

HIF-1α Regulates Osteogenesis of Periosteum-Derived Stem Cells Under Hypoxia Conditions via Modulating POSTN Expression Front Cell Dev Biol. 2022 Feb 17;10:836285. IF Mouse 1:200

Author: Available in link provided

Date of Publication : Available in link provided

Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics. Nature Communications Nat Commun. 2020 Oct;11(1):1-11 WB Human A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

HIF-1α Mediates Osteoclast-Induced Mandibular Condyle Growth via AMPK Signaling:. JOURNAL OF DENTAL RESEARCH 2020 Jun 29 WB Mouse 1:300 bone marrow monocyte (BMM)

Author: Available in link provided

Date of Publication : Available in link provided

Enhancement of bone regeneration with the accordion technique via HIF‐1α/VEGF activation in a rat distraction osteogenesis model. Journal of Tissue Engineering and Regenerative Medicine 2017 Nov 17 WB Rat bone

Author: Available in link provided

Date of Publication : Available in link provided

Heparanase from triple‑negative breast cancer and platelets acts as an enhancer of metastasis. INTERNATIONAL JOURNAL OF ONCOLOGY 2020 Aug 27 WB Human 1 : 2000 MDA-MB-231 cell

Author: Available in link provided

Date of Publication : Available in link provided

Adiponectin alleviates the symptoms of ischemic renal disease by inhibiting renal cell apoptosis. LIFE SCIENCES Life Sci. 2021 Jan;265:118825 IHC Mouse 1:200 Kidney

Author: Available in link provided

Date of Publication : Available in link provided

Differential susceptibility of PC12 and BRL cells and the regulatory role of HIF-1α signaling pathway in response to acute methylmercury exposure under normoxia. TOXICOLOGY LETTERS Toxicol Lett. 2020 Oct;331:82 WB Rat PC12 cell,BRL cell

Author: Available in link provided

Date of Publication : Available in link provided

Partial deficiency of HIF-1α in chondrocytes effected bone repair of mandibular condylar neck. ARCHIVES OF ORAL BIOLOGY Arch Oral Biol. 2021 Feb;122:105023 IHC Mouse 1:200 Chondrocytes

Author: Available in link provided

Date of Publication : Available in link provided

Sprayed copper peroxide nanodots for accelerating wound healing in a multidrug-resistant bacteria infected diabetic ulcer. Nanoscale Nanoscale. 2021 Oct;13(37):15937-15951 WB Human HUVECs

Author: Available in link provided

Date of Publication : Available in link provided

PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Jan;362:252 WB Rat 1:500 corpus cavernosum smooth muscle cells (CCSMCs)

Author: Available in link provided

Date of Publication : Available in link provided

CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma. International Journal of Nanomedicine Int J Nanomed. 2020; 15: 10401–10416 IHC，IF Mouse MV3 cell-Xenograft

Author: Available in link provided

Date of Publication : Available in link provided

Grade-targeted nanoparticles for improved hypoxic tumor microenvironment and enhanced photodynamic cancer therapy. Nanomedicine 2021 Feb 03 IF Mouse MDA-MB-231-Xenograft

Author: Available in link provided

Date of Publication : Available in link provided

Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB，IF Human MKN-45 cells

Author: Available in link provided

Date of Publication : Available in link provided

HIF‐1α facilitates osteocyte‐mediated osteoclastogenesis by activating JAK2/STAT3 pathway in vitro. JOURNAL OF CELLULAR PHYSIOLOGY 2019 Apr 29 IF Mouse 1:200 MLO-Y4 cell

Author: Available in link provided

Date of Publication : Available in link provided

Dissecting the roles of Ephrin-A3 in malignant peripheral nerve sheath tumor by TALENs. ONCOLOGY REPORTS Oncol Rep. 2015 Jul;34(1):391-398 WB Human ST88-14 cell, sNF96.2 cell

Author: Available in link provided

Date of Publication : Available in link provided

CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway. Oncology Letters Oncol Lett. 2021 Mar;21(3):1-1 WB Human 1:1000 AGS cell

Author: Available in link provided

Date of Publication : Available in link provided

Cynaroside prevents macrophage polarization into pro-inflammatory phenotype and alleviates cecal ligation and puncture-induced liver injury by targeting PKM2/HIF-1α axis. FITOTERAPIA Fitoterapia. 2021 Jul;152:104922 WB Mouse Liver

Author: Available in link provided

Date of Publication : Available in link provided

Hypoxia promotes 786-O cells invasiveness and resistance to sorafenib via HIF-2α/COX-2. MEDICAL ONCOLOGY Med Oncol. 2015 Jan;32(1):1-9 WB Human 786-O cell

Author: Available in link provided

Date of Publication : Available in link provided

Nickel-Smelting Fumes Increased the Expression of HIF-1α through PI3K/ERK Pathway in NIH/3T3 Cells. JOURNAL OF OCCUPATIONAL HEALTH 2016 Aug 04 IHC Rat lung

Author: Available in link provided

Date of Publication : Available in link provided

High-purity magnesium pin enhances bone consolidation in distraction osteogenesis model through activation of the VHL/HIF-1α/VEGF signaling:. JOURNAL OF BIOMATERIALS APPLICATIONS J Biomater Appl. 2020;35(2):224-236 IHC Rat femurs

Author: Available in link provided

Date of Publication : Available in link provided

Adiponectin alleviates the symptoms of ischemic renal disease by inhibiting renal cell apoptosis. LIFE SCIENCES Life Sci. 2021 Jan;265:118825 IHC Mouse 1:200 Kidney

Author: Available in link provided

Date of Publication : Available in link provided

Small extracellular vesicles derived from hypoxic mesenchymal stem cells promote vascularized bone regeneration through the miR-210-3p/EFNA3/PI3K pathway Acta Biomaterialia Jiawen Si WB Rat

Author: Available in link provided

Date of Publication : Available in link provided

Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways Regenerative Biomaterials Dayun Yang WB,IHC Human,Mouse lung tissues HuH7 cell,HepG2 cell

Author: Available in link provided

Date of Publication : Available in link provided

Decreasing expression of HIF-1α, VEGF-A, and Ki67 with efficacy of neoadjuvant therapy in locally advanced cervical cancer. Tingkuan Zhao IHC Human 1:200 locally advanced cervical cancer (LACC) tissue,normal cervical tissue

Author: Available in link provided

Date of Publication : Available in link provided

Mailuoning oral liquid attenuates convalescent cerebral ischemia by inhibiting AMPK/mTOR-associated apoptosis and promoting CREB/BDNF-mediated neuroprotection. Ninghua Tan WB Rat cortex tissue PC-12 cells

Author: Available in link provided

Date of Publication : Available in link provided

Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway. BRITISH JOURNAL OF CANCER Jin Zhou WB Human 1:1000 MKN45 cell,N87 cell

Author: Available in link provided

Date of Publication : Available in link provided

A bone-targeting near-infrared luminescence nanocarrier facilitates alpha-ketoglutarate efficacy enhancement for osteoporosis therapy Acta Biomaterialia Chunan Cheng IF,WB Mouse 1:200,1:1000 RAW264.7 cell,MC3T3-E1 cell

Author: Available in link provided

Date of Publication : Available in link provided

Biomimetic Hydrogel Containing Copper Sulfide Nanoparticles and Deferoxamine for Photothermal Therapy of Infected Diabetic Wounds Advanced Healthcare Materials Haijun Shen IHC Mouse 1:100 skin tissue

Author: Available in link provided

Date of Publication : Available in link provided

Hypoxia Aggravates Neuron Ferroptosis in Early Brain Injury Following Subarachnoid Hemorrhage via NCOA4-Meditated Ferritinophagy Antioxidants Zixuan Yuan WB,IF Mouse 1:1000,1:200 brain tissue HT22 cell

Author: Available in link provided

Date of Publication : Available in link provided

Manganese overexposure results in ferroptosis through the HIF-1α/p53/SLC7A11 pathway in ICR mouse brain and PC12 cells ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jian Chen WB Rat PC12 cell

Author: Available in link provided

Date of Publication : Available in link provided

Topical frankincense treatment for frostbite based on microcirculation improvements JOURNAL OF ETHNOPHARMACOLOGY Zhao Zhang IHC Rat skin tissue

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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