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Primary Antibodies
HIF-1α Antibody
HIF-1α Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: R12-2180
Reactivity:Human,Mouse,Rat
Applications:IF/ICC,WB,IHC-p,IP,ELISA
Modification:Unmodified/Total
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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HIF-1α Antibody

1) Input: Hela Lysate 2) IP product: IP dilute 1: 200 Hela treated with 0.05mM CoCl2 for 6 hours Western blot analysis: primary antibody : 1:1000 Secondary antibody: Goat anti-Mouse IgG(SA0002), 1: 5000

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Telephone
408-747-0185
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408-747-0145
Email
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Product Details
Product Name
HIF-1α Antibody
Description
hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Catalog No
R12-2180
Reactivity
Human, Mouse, Rat
Applications
IF/ICC, WB, IHC-p, IP, ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000.IP 1:200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma
Molecular Weight (Da)
92670
Gene Name
HIF1A
Protein Name
Hypoxia-inducible factor 1-alpha
Human Gene ID
3091
Human Swiss Prot No.
Q16665
Immunogen
The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
Specificity
HIF-1α Polyclonal Antibody detects endogenous levels of HIF-1α protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (26)
circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop

Author: Li, Xiaomin, Wang, Jianjun, Lin, Weihao, Yuan, Qinzi, Lu, Yanxia, Wang, Haowei, Chen, Yujia, Chen, Lixia, Dai, Peiling, Long, Huaicheng, Li, Xuenong

Date of Publication : 2022-06-23

Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis

Author: Chen Guang-yao, Liu Xiao-yu, Luo Jing, Yu Xin-bo, Liu Yi, Tao Qing-wen

Date of Publication : 2022-06-08

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p

Author: Yuexia Wang, Yue Wang, Xue Wang, Yuan Ma, Zhaojin Li, Yu Di, Yuexia Wang, Yue Wang, Xue Wang, Yuan Ma, Zhaojin Li, Yu Di

Date of Publication : 2022-04-04

Sprayed copper peroxide nanodots for accelerating wound healing in a multidrug-resistant bacteria infected diabetic ulcer

Author: Ran Zhang, Guhua Jiang, Qianqian Gao, Xiaona Wang, Yilin Wang, Xin Xu, Wenjing Yan, Haijun Shen

Date of Publication : 2021-08-25

Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment

Author: Chenghao Li, Jiawei Li, Yan Li, Ling Li, Yali Luo, Junjie Li, Yiming Zhang, Yanru Wang, Xiuzhu Liu, Xiaotian Zhou, Hongxia Gong, Xiaojie Jin, Yongqi Liu

Date of Publication : 2021-07-16

Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway

Author: Shaorong Zhao, Yannan Geng, Lixia Cao, Qianxi Yang, Teng Pan, Dongdong Zhou, Jingjing Liu, Zhendong Shi, Jin Zhang

Date of Publication : 2021-05-15

Cynaroside prevents macrophage polarization into pro-inflammatory phenotype and alleviates cecal ligation and puncture-induced liver injury by targeting PKM2/HIF-1α axis

Author: Liuhua Pei, Yifei Le, Hang Chen, Jiafan Feng, Zhijun Liu, Ji Zhu, Cui Wang, Lin Chen, Xiaobing Dou, Dezhao Lu

Date of Publication : 2021-05-11

Grade-targeted nanoparticles for improved hypoxic tumor microenvironment and enhanced photodynamic cancer therapy

Author: Ying-kai Tao, Xiao-yang Hou, Huan Gao, Xin Zhang, Feng-mei Zuo, Yun Wang, Xin-xin Li, Guan Jiang

Date of Publication : 2021-02-03

CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway

Author: Lu Gao, Jun Li, Junyu He, Lin Liang, Zhengxi He, Chunxue Yue, Xi Jin, Gengqiu Luo, Yanhong Zhou

Date of Publication : 2021-01-07

CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma

Author: Xiaoyang Hou, Yingkai Tao, Xinxin Li, Yanyu Pang, Chunsheng Yang, Guan Jiang, Yanqun Liu

Date of Publication : 2020-12-22

Partial deficiency of HIF-1α in chondrocytes effected bone repair of mandibular condylar neck

Author: Chaoyue Hong, Yi Tang, Xiu Hu, Xiwen Song, Yun Cai, Zhaohui Song, Feiwu Kang

Date of Publication : 2020-12-08

Adiponectin alleviates the symptoms of ischemic renal disease by inhibiting renal cell apoptosis

Author: Xiaotong Zheng, Dajun Liu

Date of Publication : 2020-12-01

Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics

Author: Zhang, Yang, Wang, Xiaoyong, Chu, Chengchao, Zhou, Zijian, Chen, Biaoqi, Pang, Xin, Lin, Gan, Lin, Huirong, Guo, Yuxin, Ren, En, Lv, Peng, Shi, Yesi, Zheng, Qingbing, Yan, Xiaohui, Chen, Xiaoyuan, Liu, Gang

Date of Publication : 2020-10-27

High-purity magnesium pin enhances bone consolidation in distraction osteogenesis model through activation of the VHL/HIF-1α/VEGF signaling:

Author: Musha Hamushan, Weijie Cai, Yubo Zhang, Tengfei Lou, Shaoxiang Zhang, Xiaonong Zhang, Pengfei Cheng, Changli Zhao, Pei Han

Date of Publication : 2020-05-28

Differential susceptibility of PC12 and BRL cells and the regulatory role of HIF-1α signaling pathway in response to acute methylmercury exposure under normoxia

Author: Tingting Liu, Qianqian Gao, Bobo Yang, Changsheng Yin, Jie Chang, Hai Qian, Guangwei Xing, Suhua Wang, Fang Li, Yubin Zhang, Da Chen, Jiyang Cai, Haifeng Shi, Michael Aschner, Kwaku Appiah-Kubi, Dawei He, Rongzhu Lu

Date of Publication : 2020-05-24

A COX-2/sEH dual inhibitor PTUPB ameliorates cecal ligation and puncture-induced sepsis in mice via anti-inflammation and anti-oxidative stress

Author: Yan-Feng Zhang, Chen-Chen Sun, Jia-Xi Duan, Hui-Hui Yang, Chen-Yu Zhang, Jian-Bing Xiong, Wen-Jing Zhong, Cheng Zu, Xin-Xin Guan, Hui-Ling Jiang, Bruce D. Hammock, Sung Hee Hwang, Yong Zhou, Cha-Xiang Guan

Date of Publication : 2020-02-27

Losartan protects against intermittent hypoxia-induced peritubular capillary loss by modulating the renal renin–angiotensin system and angiogenesis factors

Author: Wu Jiqiang, Chu Yao, Jiang Zhenxiu, Yu Qin

Date of Publication : 2020-01-02

Acute Methylmercury Exposure and the Hypoxia-Inducible [... formula ...] Signaling Pathway under Normoxic Conditions in the Rat Brain and Astrocytes in Vitro

Author: Jie Chang, Bobo Yang, Yun Zhou, Changsheng Yin, Tingting Liu, Hai Qian, Guangwei Xing, Suhua Wang, Fang Li, Yubin Zhang, Da Chen, Michael Aschner, Rongzhu Lu

Date of Publication : 2019-12-18

Mandibular osteotomy‐induced hypoxia enhances osteoclast activation and acid secretion by increasing glycolysis

Author: Yi Tang, Jie Zhu, Danqing Huang, Xiu Hu, Yun Cai, Xiwen Song, Zhaohui Song, Chaoyue Hong, Zifan Feng, Feiwu Kang

Date of Publication : 2018-12-12

Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway

Author: Jiang Ruibin, Guo Yan, Chen Nipi, Gao Chengxian, Ding Zhishan, Jin Bo

Date of Publication : 2018-12-05

PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells

Author: Xiang Zhang, Fan Zhao, Jian-Feng Zhao, Hui-Ying Fu, Xiao-Jun Huang, Bo-Dong Lv

Date of Publication : 2017-11-22

Dissecting the roles of Ephrin-A3 in malignant peripheral nerve sheath tumor by TALENs

Author: Zhengguang Wang, Zhendong Liu, Bo Liu, Gengyan Liu, Song Wu

Date of Publication : 2015-07-01

Hypoxia promotes 786-O cells invasiveness and resistance to sorafenib via HIF-2α/COX-2

Author: Zhao, Chun-Xiong, Luo, Chun-Li, Wu, Xiao-Hou

Date of Publication : 2014-12-07

Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and angiogenesis, and induces apoptosis of nasopharyngeal carcinoma cells

Author: Lizhu Jiang, Peng Wang, Lili Chen, Hongyan Chen

Date of Publication : 2014-08-15

HIF-1α Regulates Osteogenesis of Periosteum-Derived Stem Cells Under Hypoxia Conditions via Modulating POSTN Expression

Author: Yu Zhuang, Zhiyang Zhao, Mengjia Cheng, Meng Li, Jiawen Si, Kaili Lin, Hongbo Yu, Yu Zhuang, Zhiyang Zhao, Mengjia Cheng, Meng Li, Jiawen Si, Kaili Lin, Hongbo Yu

Date of Publication : 44609

Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway

Author: Chengyuan Yu,

Date of Publication : 1 Yang Liu,

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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