The Tfact HSF1 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of HSF1 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ HSF1 DNA-Binding ELISA detects active HSF1 in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.
