The Tfact HSF1 (Phospho-Ser303) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of HSF1 (Phospho-Ser303) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ HSF1 (Phospho-Ser303) DNA-Binding ELISA detects active HSF1 (Phospho-Ser303) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.