The Tfact Estrogen Receptor-alpha TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of Estrogen Receptor-alpha active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ Estrogen Receptor-α DNA-Binding ELISA detects active Estrogen Receptor-α in Hela and MCF7 Nuclear Extract. The Hela and MCF7 cells were grown 3 days in DMEM with 10% FBS. Hela cells were UV-stimulated (100mJ/cm2) before harvest.