This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Catalog No
A0921
Reactivity
Human, Rat, Mouse
Applications
IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
ESR1; ESR; NR3A1; Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
Molecular Weight (Da)
66216
Gene Name
ESR1
Protein Name
Estrogen receptor
Human Gene ID
2099
Human Swiss Prot No.
P03372
Immunogen
The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Ser305. AA range:276-325
Specificity
Phospho-ERα (S305) Polyclonal Antibody detects endogenous levels of ERα protein only when phosphorylated at S305.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using Estrogen Receptor-alpha (Phospho-Ser305) Antibody. The picture on the right is blocked with the phospho peptide.
