The Eotaxin-3 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Eotaxin-3 concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0114
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
Eotaxin-3
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
Q9Y258
NCBI Gene ID
10344
Target Synonyms
CC chemokine IMAC, Macrophage inflammatory protein 4-alpha, MIP-4-alpha, Small-inducible cytokine A26, Thymic stroma chemokine-1, TSC-1
Human Eotaxin-3 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human Eotaxin-3 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Eotaxin-3. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Eotaxin-3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Eotaxin-3 proteins within the range of 32-3000 pg/ml.
