The I-TAC (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant I-TAC concentrations within any experimental sample including cell lysates, serum and plasma.
Human I-TAC recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human I-TAC was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for I-TAC. Each known sample concentration was assayed in triplicate. The OmniKine™ Human I-TAC ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human I-TAC proteins within the range of 32-2000 pg/ml.
