The VEGF (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant VEGF concentrations within any experimental sample including cell lysates, serum and plasma.
Human VEGF recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
The data and subsequent graph were obtained after performing a cytokine ELISA for VEGF. Each known sample concentration was assayed in triplicate. The OmniKine™ Human VEGF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human VEGF proteins within the range of 16-1000 pg/ml.