The IL-1beta (Murine) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant IL-1beta concentrations within any experimental sample including cell lysates, serum and plasma.
Mouse IL-1beta recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Mouse IL-1beta was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for IL-1beta. Each known sample concentration was assayed in triplicate. The OmniKine™ Murine IL-1beta ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IL-1beta proteins within the range of 63-4000 pg/ml.
