The MIP-1beta (Murine) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant MIP-1beta concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0195
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
MIP-1beta
Reactivity
Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P14097
NCBI Gene ID
20303
Target Synonyms
C-C motif chemokine 4, Immune activation protein 2, ACT2, Macrophage inflammatory protein 1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4
Mouse MIP-1beta recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Mouse MIP-1beta was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for MIP-1beta. Each known sample concentration was assayed in triplicate. The OmniKine™ Murine MIP-1beta ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine MIP-1beta proteins within the range of 4-500 pg/ml.
